Liquid breakup under the variation of AC frequency has been studied experimentally in the electrohydrodynamic atomization. The effect of parameters such as charging voltage, flow rate, nozzle tip inner diameter and power frequency have been considered. This work was performed to investigate the experimental analysis for the effect of AC frequency on breakup process, the mapping of occurrence of disintegration region, and the relationship between the applied power and the droplet radius. The experimental results show that the increase of applied voltage in a certain frequency band leads to a reduction in the droplet size within the limits from 50Hz to 400Hz. The transition phenomena from dripping mode to spindle mode were observed under the band of sudden fall of droplet radius changing ratio, and the synchronous region were produced within the range of applied voltage from 5kV to 6kV.