An experimental study was carried out in order to investigate the influence of flow conditions on a boundary layer in the near-wake of a flat plate. The flow conditions in the vicinity of the trailing edge that is influenced by upstream condition history are an essential factor that determines the physical characteristics of a near-wake. Tripping wires attached at various positions were selected to change flow conditions of a boundary layer. The flows such as laminar, transitional, and turbulent boundary layer at 0.98C from the leading edge are imposed in order to investigate the evolution of symmetric and asymmetric wake. An x-type hot-wire probe(55P61) is employed to measure at 8 stations in the near-wake. Test results show that the near-wake for the case of a turbulent boundary layer is relatively insensitive to instability after separating at the trailing edge, and Reynolds shear stress in the near-wake for the case of a turbulent boundary layer collapses due to turbulent kinetic energy.