소나무잔나비버섯 인공재배를 위한 균사배양적 특성을 조사한 결과는 다음과 같다. 1) 소나무잔나비버섯균의 적정배지는 PIDA(Pine Dextrose Agar)에서 66.3mm/10일로 균사생장과 균사밀도가 가장 좋았으며, 그 다음은 GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA(pH4.7), CHA, MEA(pH4.7) 순이었다. 2) 소나무잔나비버섯의 균사생장과 밀도에 가장 적정한 온도는 $30^{circ}C$이었으며 $40^{circ}C$에서는 균사가 사멸하였다. KNAC3005 균주는 $30^{circ}C$에서 66.3mm/10일로 균사생장과 밀도가 가장 양호하였으며, 온도별로는 25, 20, 15, 35, 10, $5^{circ}C$ 순으로 균사생장 속도와 밀도가 좋았다. 3) 소나무잔나비버섯의 균사생장과 밀도에 가장 적정한 산도(pH)는 6.0에서 88.4mm/10일이며 그보다 높거나 낮으면 균사생장과 밀도에 저해를 받는다. 4) 소나무잔나비버섯의 균사생장에 적합한 탄소원은 maltose로 331mg/25ml/15일이며, 최적 질소원은 peptone으로 347mg이다. 최적 유기산은 glutamic acid로 357mg이었다. 최적 비타민은 biotin으로 370mg/15days이며 C/N율은 40이 적정하였다.
The results of examining characteristics of mycelial growth and culture condition for determining the condition of artificially culturing Fomitopsis pinicola are as follows. 1) Mycelial growth and density of F. pinicola. were the highest in the medium of PIDA(Pine Dextrose Agar;66.3mm/10d) followed by the order of GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA(pH 4.7), CHA, and MEA(pH 4.7). 2) Optimal temperature for the mycelial growth and density of F. pinicola were shown to be $30^{circ}C$, but the mycelia were dead at $40^{circ}C$. the mycelial growth and density of KNAC9005 strains was the highest at $30^{circ}C$(66.3mm/10d) followed by the order of 25, 20, 15, 35, 10, and $5^{circ}C$. 3) Optimal pH for the mycelial growth and density of $40^{circ}C$ was revealed to be 6.0(88.4mm/10d). above or below pH 6.0, the mycelial growth and density were shown to be retarded. 4) Optimal carbon, nitrogen and organic acid sources for the spawn growth of $40^{circ}C$ were maltose(331mg/25ml/15d), peptone(347mg/25ml/15d), and glutamic acid(357mg/25ml/15d), respectively. Optimal level of biotin was 370mg/15d and optimal C/N ratio was 40.
