This paper continues companion paper, part I : measurement and analysis. As shown in companion Paper, information and energy in monaural signal is quite different from that of binaural signal. In this paper, difference between monaural and binaural signal of transportation noise are investigated in subjective response test. We executed hearing screening test before giving a subject response test and excluded subjects who had physical hearing loss. An annoyance response test was conducted using headphone to avoid cross-talk effect in binaural testing. Percentage of highly annoyed under binaural signal reproduction is higher than percentage of highly annoyed under monaural signal reproduction. Result implies binaural reproduction technique is proper for a study of human response to short-term noise exposure in a headphone simulated-environment.