The objective of this study was to investigate the environmental effects on duration time of milk flow, peak milk flow, and average milk flow in teats, and to estimate their relationships with milk yields in morning and evening milking, and to provide fimdamental information for the further study on their relationships with clinical mastitis and somatic cell in milk. A total of 6,768 milking records were studied in 72 Holstein cows. The influences of season, parity, lactation month, and milking interval on characteristics of milk flow considering in linear model were significant(p<.05). Duration of milk flow was longest at milking in fall, past first parity and second month of lactation, and with milking interval over than 13.5hrs. Average milk flow rate and peak milk flow rate were highest at milking in summer, past first parity and 8${sim}$10 months of lactation, and with milking interval over than 13.5hrs. Milk flow rate was positively correlated to milk yield, and negatively correlated to the duration of milk flow. However duration milk flow was positively correlated to the milk yield with high level of correlation coefficient(+.60). For the establishment of optimum selection criteria on these traits, other aspects such as the udder health, disease and respective economic weights of milk flow characteristics in this study must be considered to develop the indices.