Biotop map can be utilized for nature conservation and assessment of environmental impact for human activities in urban area. High resolution satellite images such as IKONOS and KOMPSAT1-EOC were interpreted to classify land use, hydrology, impermeable pavement ratio and vegetation for biotop mapping. Wildlife habitat map and detailed vegetation map obtained from former study results were used as ground truth data. Vegetation was investigated directly for the area where the detailed vegetation map is not available. All these maps were combined and the boundaries were delineated to produce the biotop map. Within the boundary, the characteristics of each polygon were identified, and named. This study investigates the possibility of biotop mapping using high resolution satellite remote sensing data together with field data with the goal of contributing to nature conservation in urban area.