The purpose of this research was to investigate the effects of supercritical fluid extract of Kamichungbieum (SFE) on the contraction of isolated rat aorta. The contractile force of rat aorta was measured with force displacement transducer under 1.5g loading tension. The contraction of aorta induced by phenylephrine 0.1 μM was inhibited by SFE. The aorta relaxed by SFE was inhibited by the pretreatment of L-NNA, ODQ or indomethacin, respectively. These results indicate that SFE induce the relaxation of isolated aorta via activation of nitric oxide, cAMP and cyclooxygenase in epithelium cells.