In order to establish the experimental clinical model for evaluating the influences on the sexual hormones of SD-rat administrated with Methoxychlor(MET), it was dissolved in acetone and olive oil (1:19), which was administrated orally at doses of 200㎎/㎏ body weight/day for 7 days. The Testosterone value was measured in sera by Gamma Count with 1251 isotype, also weights of the body, testis and liver were measured for 15 weeks. Testosterone in the serum of intacted SD-rats was 0.51±0.43ng/㎖(n=5), but it was 0.69±0.18(n=2)ng/㎖l on 1 week. 0.28±0.05ng/㎖(n=2) on 3 week, 1.52±0.95ng/㎖(n=3) on 5 week, and 0.54±1.95 ng/㎖(n=3) 15 week post-dosing MET, respectively. Histologically, the numbers of spermatozoa and Leydig cells were reduced, which might influence to reduce sexual hormone, and AST and ALT was not increased in the serum due to administrating with MET, blood profile was not changed according to the administration of MET excepting MCH(Mean Corpuscular Hemoglobin). This study suggest that change of male sexual hormone by MET be a experimental model for evaluating some drugs associate with sex hormone.