Jaeum-Dan was reported new anti-aging effect formula previously. We investigated the physiological effects on human skin with Jaeum-Dan essence (JED). We made a skin application product as essence form contained oriental herbs, and measured various physiological effects such as skin moisture contents, red blood cell flow rates, skin surface temperature, corneocyte exfoliation and skin intensity when volunteers(female, n=40) used the application product for 4 weeks. The results were as follows: The skin moisture contents were not only increased in human face skin through using the product, but also red blood cell flow :rates in face skin were increased. After using the essence, the face skin temperature rose and corneocyte exfoliation was decreased. Furthermore the upper dermis intensity of skin in female face was subsequently increased 4 weeks later. The results indicate that the application product containing JED was improved the physiological effects of human facial skin..