The vibration analysis of an axially moving membrane are investigated when the membrane has the two sets of in-plane boundary conditions, which are free and fixed constraints in the lateral direction. Since the in-plane stiffness is much higher than the out-of-plane stiffness, it is assumed during deriving the equations of motion that the in-plane motion is in a steady state. Under this assumption, the equation of out-of-plane motion is derived, which is a linear partial differential equation influenced by the in-plane stress distributions. After discretizing the equation by using the Galerkin method, the natural frequencies and mode shapes are computed. In particular, we put a focus on analyzing the effects of the in-plane boundary conditions on the natural frequencies and mode shapes of the moving membrane.