In this study, to investigate the effects of omitting low-amplitude cycles from a flight-simulation loading, crack growth tests were conducted on 2124-T851 aluminum alloy specimens. Three test spectra were generated by omitting small load ranges as counted by the rain-flow count method. The crack growth test results were compared with the data obtained from the flight-simulation loading. The experimental results show that the ranges equal to or smaller than 5% of the maximum load do not contribute to crack growth behavior because these are below the initial stress intensity factor range. Omitting these from the flight-simulation loading, test time can be reduced by 54%. However, in the case of omitting the load ranges below 15% of the maximum load, crack growth rates decreased, and crack growth curve deviated from the crack growth data under the flight-simulation loading because loading cycles above fatigue fracture toughness were omitted.