Waste concentration and waste generation rate in seawater aquaria by common conger (Astrocongey myriaster) and olive flounder (Paralichyhus olivaceus) were investigated. The initial fish density of common conger and olive flounder were $3{\%};and ;2.8{\%}$ of total weight of aquarium water, respectively Wastes in the seawater aquaria, such as protein, total suspended solid (TSS), ammonia, turbidity and chemical oxygen demand (COD) increased with days after stocking. Protein generation rate of common conger increased until 1 day after stocking while that of olive flounder increased until 3 days. The average protein generation rates of common conger and olive flounder were $0.28g/kg{cdot}day;and;0.21g/kg{cdot}day$, respectively. The trends of other waste generation rate were similar to protein generation rate.