Physiological responses and tolerance limit of hybrid striped bass $(Morone;Chrysops;male{ imes};M.;saxatilis;female),$ were investigated on salinity changes from 33 psu to 0 psu and from 0 to psu 33 psu in two separate trials, respectively. In the acute salinity decrease from sea water (33 psu) to fresh water (0 psu), plasma level of cortisol, glucose, total protein, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected, however, $Na^+,;Cl^-$ and osmolality significantly decreased. In the acute salinity increase from fresh water to sea water (33 psu), plasma cortisol level did not change. Glucose and total protein concentrations in plasma and hematocrit (Hct) decreased, whereas AST, ALT, $Na^+,;Cl^-$ and osmolality increased. The hybrid striped bass can overcome the acute changes of salinity, from 0 psu to 33 psu or from 33 psu to 0 psu, But salinity decrease stressed the fish much more than salinity increase.