Objectives : In order to develop the measuring tool of visual display terminal (VDT) syndrome and investigate the variables affecting the development of VDT syndrome. Methods : A questionnaire study accompanied with the evaluation of working environment was performed with 42 VDT users from banking operations. Results : As a results of analysis with data colleted by newly developed questionnaire, VDT syndrome included five factors named as eye-related component, and psychological component. general body discomfort component. musculoskeletal component, and skin-related component. The scores of eye-related symptoms was 15.17, scores of psychological symptoms was 7.36, scores of general body discomfort symptoms was 10.52, scores of musculoskeletal symptoms was 9.38, scores of skin-related symptoms was 3.67, and total scores was 46.10. Scores of 2.05 eye fatigue in the level of eye-related symptoms were higher significantly. Scores 50.52 of female was statistically higher than scores 41.67 of male. Conclusions : The subjective symptoms of VDT syndrome scores was significantly associated with gender and age.