This study examined the muscle fatigue of the erector spinae according to box size during lifting activity. Ten healthy adult man participated in the experiment for lifting the boxes 10 times with 0.2 lift/s frequency. Bilateral surface EMG (Electomyogtamphy) recordings were made regarding M. (Muscle) Longissimus, M. Iliocostal and M, Multifidus activity throughout lifting working. The median frequency in back muscle fatigue was investigated using power spectral analysis of surface EMG. When the box larger than shoulder width was lifted, the muscle fatigue of M, Multifidus was shown larger than one of the other muscles. Accordingly, the result far M. Multifidus can be applied in optimizing design parameter of box. Also, the standard of box width for box makers have to be below about 420 mm, being average shoulder width of adult male.