A microdialysis coupled flow injection amperometric $({mu}FIA)$ biosensor was calibrated to measure the concentration of sucrose using 11 standard samples from 2 ml to 70 ml of sucrose solution. The output of the sensor increased linearly with an increase in the sucrose concentration with an $r^2$ correlation of 0.99. The amperometric biosensor was then applied to measure the sucrose concentration of 4 commercial samples (Orange and Pineapple juices, Pepsi, Sprite) and the results compared with those by HPLC. Around $20~30\%$ error was observed in sucrose concentration measurements of the samples analyzed. The sensor has potential in rapid measurement once the calibration is done. Potential for on-line sensing is also discussed.