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Cryopreservation of Zygotic Embryos of Herbaceous Peony (Paeonia lactiflora Pall.) by Encapsulation-Dehydration
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  • Cryopreservation of Zygotic Embryos of Herbaceous Peony (Paeonia lactiflora Pall.) by Encapsulation-Dehydration
  • Cryopreservation of Zygotic Embryos of Herbaceous Peony (Paeonia lactiflora Pall.) by Encapsulation-Dehydration
저자명
Kim. Hyun-Mi,Shin. Jong-Hee,Sohn. Jea-Keun
간행물명
Korean journal of crop science
권/호정보
2004년|49권 4호|pp.354-357 (4 pages)
발행정보
한국작물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A simplified technique which cryoprotects zygotic embryos by encapsulation-dehydration was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest survival rate $(85\%)$ was obtained from embryos treated by encapsulation-dehydration. The zygotic embryos were precultured on MS medium containing 0.3mg/L $GA_3$ for 1 day. The precultured embryos were encapsulated in $3\%$ (w/v) alginate beads and immersed for 1 h in MS medium containing 2 M glycerol and 0.5 M sucrose. The encapsulated embryos were dehydrated for 5h by air drying prior to direct immersion in liquid nitrogen. This encapsulation-dehydration method appears to be a promising technique for germplasm cryopreservation of a herbaceous peony.