This research investigates dynamic characteristics of a linear motion (LM) guide through a experimental result and theoretical analysis. The stiffness in the LM guide is determined by the preloading due to the minus clearance between the ball bearing and the contact surface and it can be derived by Hertzian contact theory and the nonlinear motion of equation. The vibration analysis is performed using Lagrange equations and its result agrees with the experiment result. Using the sensitivity analysis on design parameters such as the contact angles of ball bearings and the eccentricity of mass center, the variation of the natural frequencies can be predicted.