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Efficient Isolation of Intact RNA from the Soft Coral Scleronephthya gracillimum $(Kddot{u}kenthal)$ for Gene Expression Analyses
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  • Efficient Isolation of Intact RNA from the Soft Coral Scleronephthya gracillimum $(Kddot{u}kenthal)$ for Gene Expression Analyses
  • Efficient Isolation of Intact RNA from the Soft Coral Scleronephthya gracillimum $(Kddot{u}kenthal)$ for Gene Expression Analyses
저자명
Woo. Seo-Nock,Yum. Seung-Shic,Yoon. Moon-Geun,Kim. Sa-Heung,Lee. Jong-Rak,Kim. Jeong-Ha,Lee. Taek-Kyun
간행물명
Integrative biosciences
권/호정보
2005년|9권 4호|pp.205-209 (5 pages)
발행정보
한국동물학회
파일정보
정기간행물|ENG|
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기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A rapid, simple and efficient method to extract RNA from the adult polyps of a soft coral, marine cnidarian, Scleronephthya gracillimum $(Kddot{u}kenthal)$; was developed in this study. The highest yield and purity of RNA was obtained with the lysis solution containing 35 mM EDTA, 0.7 M LiCl, 7.0% SDS, and 200 mM Tris-Cl (pH 9.0). Approximately $40{mu}g$ of total RNA was extracted from 200 mg of liquid nitrogen-pulverized polyp tissue. The ratio of absorbance at 260 nm and 280 nm ranged from 1.8 to 2.0. The results of the reverse transcription polymerase chain reaction (RTPCR) with ${eta}-actin$ gene specific primers and Northern blot analysis using the same gene probe revealed that the RNA extracted by our method had high quality, and was sufficient for subsequent molecular biological analyses. This method was effective for RNA extraction from other soft coral species which belong to the genus Dendronephthya.