Purpose: The purpose of this study was to evaluate effect of kinesio taping on the delayed onset muscle soreness(DOMS). Methods Fourteen healthy subjects were randomly divided into two groups; experimental group(n=7) and control group(n=7). All subjets performed eccentric exercise of knee extensor until exhausted. After 24 hours experimental group was taped with kinesio tape to the quadriceps muscle and control group was not applied. To compare with the effectiveness of kinesio taping between two groups, I measured DOMS with pain(VAS) and temperature(DITI). The data were analyzed by Independence T-test. Results: The Experimental group was not significantly different the body temperature and pain than Control group at 24 hours after exercise without taping. The Experimental group was more decreased pain and temperature than Control group at 24 hours after exercise with taping. The Experimental group was more decreased pain than Control group at 48 hours after exercise. The Experimental group was more decreased pain and temperature than Control group at 72 hours after exercise. Conclusion: Experimental group more rapidly recovered temperature and more rapidly decreased pain after apply taping than control group.