The purpose of this study was to measure magnitude of lower leg muscle activity during dynamic stability tests performed on an unstable board by subjects with sprained lateral ankles. Fifteen lateral ankle sprain subjects(8 male, 7 female) participated in this study. The muscle activity was measured at gastrocnemious, tibialis anterior, peroneus longus during dynamic stability tests performed on Biodex stability system as surface EMG. The EMG amplitude of each muscle was normalized to the amplitude in the maximal voluntary isometric contraction(MVIC) of each muscle. As results, peroneus longus evidenced significant difference at stable and unstable grade comparing injured with uninjured sides. Gastrocnemious and tibialis anterior evidenced no significant difference. Change of muscle activity with stable and unstable grade evidenced no significant difference on injured and uninjured sides. The data provided information on peroneus longus contributes to dynamic stability after lateral ankle sprains. Peroneus longus training program may have to be emphasized after an ankle sprain.