Instantaneous frequency of doppler signals is used to get the information of the relative velocity and the miss distance between a missile and a corresponding target. In this paper, we have performed time-frequency analysis and instantaneous frequency estimation with Short Time Fourier Transform(STFT), Wigner Ville Distribution(WVD) and Continuous Wavelet Transform(CWT) about the doppler signals generated by moving targets. Performance evaluation was performed using simulated doppler signals generated by a single moving target and two moving targets. From the results of the time-frequency analysis, we found that WVD method was the most efficient instantaneous frequency estimator among the three methods. But in case of two moving targets, WVD method got cross talks and CWT method got oscillation when two doppler frequencies were close to each other.