Objectives : The aim of this study is to investigate the effect of Shinmun-acupuncture stimulation on the relationship of change in electrocardiopgraphy (ECG). Methods : For this purpose, 11 healthy volunteers were acupunctured at Shinmun acupoint using the reinforcing or reducing by inserting the needle in the same direction as the channel runs or in the opposite direction (迎隨補瀉). Then, we measured and observed the change of standard leads I, II and III in ECG. Results : In lead I , acupuncture treated groups were increased the activity of PR interval and PR segment compared to the control group. In lead II, during the acupuncture treated group by inserting the needle in the opposite direction as the channel runs was increased the activity of T wave duration, and after the acupuncture group by inserting the needle in the same direction as the channel runs was increased the activity of PR interval. In lead III, acupuncture treated groups were increased the activity of ST duration, PR interval and QT interval. Conclusions : These results suggested that Shinmun acupuncture stimulation plays an important role on the activities of ECG.