The purpose of this study was to investigate the possibility of Inonotus obliquus as an functional resource. To assess the effects of Inonotus obliquus in culturing method by using unpolished rice in 15non-insulin dependent diabetes mellitus persons, we examined changes of hematological and chemical variables, DM indices content in serum during the Inonotus obliquus supplementation. GOT, GPT concentrations were significantly decreased. But TIBC content was significantly increased. Fasting glucose level during Inonotus obliquus supplementation was decreased respectively. These results show that modest dose of Inonotus obliquus in culturing method by using unpolished rice supplementation can decrease fasting blood glucose level without any changes in health indices and nutrition status of the non-insulin dependent diabetes mellitus person. These results indicated that Inonotus obliquus in culturing method by using unpolished rice diet is effective therapeutic regimen for the control of metabolic derangements in diabetes mellitus. Also, the results imply that Inonotus obliquus in culturing method by using unpolished rice can be used as possible functional food materials. However, large amounts of Inonotus obliquus in culturing method by using unpolished rice showed be used cautiously application.