The effects of chloroform extract from Riccardia marginata on antifungal activity were investigated. The crude chloroform extract of R. marginata inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (4 mm inhibition zone at $150;{mu}g/disc$) and the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (6 mm inhibition zone at $150;{mu}g/disc$), and inactive to P388 murine leukaemia cells ATCC CCL 46 P388D1, ($IC_{50};>25,000;{mu}g/mL$ at $150;{mu}g/disc$). This crude chloroform extract of R. marginata showed strong antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes.