Objectives : This study was conducted to investigate the effects Innotus obliquus on the cytokine expression in Raw 264.7 cells. Methods : In Raw 264.7 cells stimulated with lipopolysaccaride(LPS) to Mimic inflammation, Innotus obliquus ethanol extract inhibited nitric oxide production in a dose-dependent manner and abrogated inducible nitric oxide sythase(iNOS) and cyclooxigenase (COX-2). The levels of cytokines were analyzed by sandwich immuno assays. Results and Conclusions : Results provided evidence that Innotus obliquus inhibited the production of IL-b, and IL-6 in Raw 264.7 cells activated with lipopolysaccharide (LPS). These finding suggested that Innotus obliquus can produce anti-inflammatory effect, which may playa role in adjunctive therapy in Gram-negative bacterial infections.