Methods : This study was conducted to carry out quantitative evaluation using HPLC Content analysis was done using HPLC Results : According to HPLC analysis, each BVA-1 contained approximately $0.36{mu}g$ melittin, and BVA-2 contained approximately $0.54{mu}g$ melittin. But the volume of coating was so minute, slight difference exists between each needle. Conclusions : Above results indicate that the bee venom acupuncture can complement shortcomings of syringe usage as a part of Oriental medicine treatment, but extensive researches should be done for further verification.