꽃마리를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. EtOAc와 n-BuOH 분획에 대하여 column chromatography를 반복하여 4종의 flavonol 배당체를 분리하였다. 각각에 대하여 2D-NMR을 포함한 스펙트럼 데이터의 해석과 문헌 자료를 조사하여 $kaempferol-3-O-{eta}-{D}-glucopyranoside(astragalin),;kaempferol-3-O-{alpha}-{L}-rhamnopyranosyl(1{ ightarrow}6)-{eta}-{D}-glucopyranoside(nicotiflorin),;quercetin-3-O-{alpha}-{L}-rhamnopyranosyl(1{ ightarrow}6)-{eta}-{D}-glucopyranoside(rutin),;quercetin-3-O-{eta}-{D}-glucopyranoside(isoquercitrin)$로 구조를 결정하였다. 이 화합물들은 꽃마리에서는 이번에 처음 분리, 보고되었다. 또한 $nicotiflorin(100;{mu}g/ml)$은 hACAT1에 대하여 $68.3{pm}1.2%$ 저해활성을 나타내었다.
The MeOH extracts obtained from whole plant of Trigonotis peduncularis Benth. were solvent fractionated using EtOAc, n-BuOH and water, successively. The EtOAc and n-BuOH fractions gave four flavonol glycosides through application of silica gel and octadecyl silica gel (ODS) column chromatographies. The chemical structures of the flavonol glycosides were determined by the interpretation of several spectral data including 2D-NMR as $kaempferol-3-O-{eta}-{D}-glucopyranoside;(astragalin,;1),;kaempferol-3-O-{alpha}-{L}-rhamnopyranosyl;(1{ ightarrow}6)-{eta}-{D}-glucopyranoside;(nicotiflorin,;2),;quercetin-3-O-{alpha}-{L}-rhamnopyranosyl(1{ ightarrow}6)-{eta}-{D}-glucopyranoside;(rutin,;3),;quercetin-3-O-{eta}-{D}-glucopyranoside;(isoquercitrin,;4)$. The flavonoids have been first isolated from this plant. Nicotiflorin $(100;{mu}g/ml)$ showed $68.3{pm}1.2%$ of the inhibitory effect on hACAT1(human Acyl CoA: cholesterol transferase 1) activity.
