This study investigated the effect of anticurvature filing method on preparation of the curved root canal using ProFile. Thirty six resin blocks were divided equally into three groups by instrumentation motions: anticurvature filing motion. circumferential filing motion and straight up-and-down motion. Each resin block was sectioned at 8mm level from the apex and at the greatest curvature of the canal and reassembled in metal mold by a modified Bramante technique. All groups were instrumented with the ProFile system. At each levels. image of sectioned surface were taken using CCD camera under a stereomicroscope at $ imes40$ magnification and stored. Distances of transportation at the inner and outer area of curvature and the centering ratio were determined and compared by statistical analysis. along with the assessment of the increase of root canal cross-sectional area. The results were as follows; 1. In all groups. there was no statistical difference in the mean increase of root canal cross-sectional area. the centering ratio. and the mean distances of transportation at the inner area of curvature at each level. 2. At 8mm level from the apex. the mean distances of transportation at the outer area of curvature decreases in following order anticurvature filing motion. circumferential filing motion. straight up-and­down motion but. no significant difference at the greatest curvature of the canal among three groups. Effect of anticurvature filing motion using ProFile does not seem to be different from other instrumentation motions at the inner area of curvature in curved root canal.