Chitosan, a poly-saccharide, has been widely studied as bone substitution and membrane material in periodontology as well as orthopedic application. Tetracycline has been considered for use in the treatment of chronic periodontal disease and gingivitis. The aim of the present study is to make a comparative study of two ways for fabricating chitosan membrane containing tetracycline and achieve sustained and controlled local delivery of the antibiotic to the diseased tissue. Two types of membranes, non-woven and sponge, were fabricated with various concentration of tetracycline. The tensile strength of the chitosan fiber was tested by Vibrodyn and the membranes were tested on a universal test machine. Streptococcus mutans were employed to examine the antibacterial properties of the membranes. Body-safety of the membranes were determined by the Salmonella Mutagenicity test, the micronucleus test, the acute oral toxicity test, and skin patch test. The sponge type chitosan membrane was found to have an excellent tensile strength.