The cultivation of akoya pearls was carried out with bio bead nucleus prepared using the hydroxyapatite. The akoya pearls cultured with bio bead nucleus were shown the color of cream. The luster and orient effect created by an action of the light from nacre were magnificent. The result of XRD is found that the nacre is a calcium carbonate of aragonite form. From the result of SEM, it is found that layers of calcium carbonate and conchiolin are stratified. The properties of akoya pearls cultured with bio bead nucleus are same those of akoya pearl cultivated with bead nucleus made from washboard shell. Because of the bio bead nucleus developed in this study has such a high capacity in producing various sizes and shapes, it is very advantageous for the cultivation of akoya pearls.