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Simultaneous Determination of Cannabidiol, Cannabinol, and ${Delta}^9-Tetrahydrocannabinol$ in Human Hair by Gas Chromatography-Mass Spectrometry
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  • Simultaneous Determination of Cannabidiol, Cannabinol, and ${Delta}^9-Tetrahydrocannabinol$ in Human Hair by Gas Chromatography-Mass Spectrometry
  • Simultaneous Determination of Cannabidiol, Cannabinol, and ${Delta}^9-Tetrahydrocannabinol$ in Human Hair by Gas Chromatography-Mass Spectrometry
저자명
Kim. Jin Young,Suh. Sung Ill,In. Moon Kyo,Paeng. Ki-Jung,Chung. Bong Chul
간행물명
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
권/호정보
2005년|28권 9호|pp.1086-1091 (6 pages)
발행정보
대한약학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

An analytical method was developed for evaluating the cannabidiol (CBO), cannabinol (CBN), ${Delta}^9-tetrahydrocannabinol$ $({Delta}^9-THC)$ level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50mg) were washed with isopropyl alcohol and cut into small fragments (< 1mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0M NaOH for 10 min at $95^{circ}C$. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and ${Delta}^9-THC$ at three concentration levels were $37.9-94.5\%$ with good correlation coefficients $(r^2>0.9989)$. The intra-day precision and accuracy ranged from $-9.4\%;to;17.7\%$, and the inter-day precision and accuracy ranged from $-15.5\%;to;14.5\%$, respectively. The limits of detection (LOD) for CBD, CBN, and ${Delta}^9-THC$ were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated.