This study was investigated to compare the suppression of Meloidogyne arenaria by different treatments of Pasteuria penetrans which is known for biological control agent against Meloidogyne spp.. In order to select proper number of P. penetrans showing good suppression effect, P. penetrans were mixed with M, arenaria for attachment using three different concentration such as $3{ imes}10^4$, $3{ imes}10^5$ and $3{ imes}10^6$ endospores/5 g medium, followed by treating them onto the roots of tomato. After 14 weeks incubation, P. penetrans at $3{ imes}10^6$ endospores showed highest activity against the formation of gall caused by M, arenaria. At a dose of $3{ imes}10^5$ endospores/5 g medium, P. penetrans was treated into soil either mixing with soil or spray onto soil surface for comparing of suppressive efficacy. When the antagonistic bacterium was treated by the former method, it suppressed more effectively Using P. penetrans at $3{ imes}10^6$ endospores and mixing with soil method, suppression was compared among P. penetrans, $PASTORIA^{(R)}$(Japan) and $Fosthiazate^{(R)}$(Korea). P. penetrans was more potent than $PASTORIA^{(R)}$(Japan) and as similar as $Fosthiazate^{(R)}$(Korea). Therefore, these results suggested that P. penetrans can be used for controling of M. arenaria as biological control agent. Furthermore, thess results can be provided to develop environmentally-friendly nematicide.