An IDI diesel engine used to agricultural tractors was fueled with $20\%$ biodiesel fuel(BDF 20) in excess of 300 hours. Engine dynamometer testing was completed at regularly scheduled intervals to monitor the engine performance and exhaust emissions. The engine performance and exhaust emissions were sampled at 1 hour interval for analysis. The combustion variation such as the combustion maximum pressure and the crank angle at this maximum pressure was not appeared during long-time dynamometer testing. Also, BSFC with BDF 20 resulted in lower than with diesel fuel. Since the biodiesel fuel used in this study includes oxygen of about $11\%$, it could influence the combustion process strongly. So, BDF 20 resulted in lower emissions of carbon monoxide, carbon dioxide, and smoke emissions without special increase of oxides of nitrogen than diesel fuel. It was concluded that there was no unusual deterioration of the engine, or any unusual change in exhaust emissions from using the BDF 20.