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Surface Characteristics and Fibroblast Adhesion Behavior of RGD-Immobilized Biodegradable PLLA Films
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  • Surface Characteristics and Fibroblast Adhesion Behavior of RGD-Immobilized Biodegradable PLLA Films
  • Surface Characteristics and Fibroblast Adhesion Behavior of RGD-Immobilized Biodegradable PLLA Films
저자명
Jung. Hyun Jung,Ahn. Kwang-Duk,Han. Dong Keun,Ahn. Dong-June
간행물명
Macromolecular research
권/호정보
2005년|13권 5호|pp.446-452 (7 pages)
발행정보
한국고분자학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLA-g-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/$cm^2$. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control<PLLA-g-PAA=PLLA-g-GRDG<PLLA-g-GRGD, indicating that PLLA-g-GRGD has the highest cell adhesive property.