This paper aims to analyze the acoustic effects of vowels produced in a distant-talking environment. The analysis was performed using a statistical method. The influence of gender and speakers on the variation was also examined. The speech data used in this study consist of 500 distant-talking words and 500 normal words of 10 speakers (5 males and 5 females). Acoustic features selected for the analysis were the duration, the formants (Fl and F2), the fundamental frequency and the total energy. The results showed that the duration, F0, F1 and the total energy increased in the distant-talking speech compared to normal speech; female speakers showed higher increase in all features except for the total energy and the fundamental frequency. In addition, speaker differences were observed.