Invasive aspergillosis is the most common Aspergillus fumigatus infection in immunocompromised patients. Although the treatment of the invasive aspergillosis has been mostly relied on antifungal agents, there still exists the need for more effective therapy. To develop cellular immunotherapy specific for Aspergillus fumigatus, we generated specific T cells using dendritic cells (DCs) pulsed with an Aspergillus fumigatus derived recombinant protein in vitro and examined their functions. The f16. p2+3 region containing the conserved region of Asp f16 gene was cloned from Aspergillus fumigatus and the recombinant protein was produced in E. coli. $IFN-{gamma}$ secretion from the T cells stimulated with recombinant f16. p2+3 (rf16. p2+3) was measured by enzyme linked immunospot(ELISPOT) assay and the cytolytic activity of the stimulated T cells by $^{51}Cr$ release assay. The number of $IFN-{gamma}$ secreting cells were significantly increased in the peripheral blood mononuclear cells (PBMCs) stimulated with the rf16. p2+3 pulsed DCs $(31{pm}12;spots/10^4 cells)$, compared to that of PBMCs directly stimulated with rf16. p2+3 $(83{pm}15; spots/10^6 ;cells)$. $IFN-{gamma}$ ELISPOT assay using purified CD4+ or CD8+ as responder cells showed that CD4+ T cells $(43; spots/10^4; cells)$ mainly produced $IFN-{gamma}$ compared with CD8+ T cells $(7 spots/10^4; cells)$. Furthermore, helper T cells specific for rf16. p2+ 3 could be efficiently generated by the stimulation with DCs for two weeks. However, cytotoxic T lymphocyte activity was not induced. Our results suggest that the rf16. p2+3 protein could be used for the gene ration of helper T cells in vitro.