The exposed strain of C. riparius treated with di (ethyl- hexyl)-phthalate (DEHP) did not result in a consistent relationship between mortality or sex ratio and chemical concentrations. And after treating with DEHP, the emergent female from the exposed strain appeared to be fatty with a large body volume comparing with the non - exposed strain. The emergent period (EP) was especially different between the exposed fourth strain and the non-exposed strain; generally the exposed strain was $7~10$ days, and non - exposed strain was $17~24$ days. Regarding sustainable exposed effects, the EP, the first emergent day (FED), and the body volume (BV) could be suggested as suitable bio - markers for detecting of exposure to various EDCs.