This study investigated the effects of polymannuronate feeding on cholesterol levels of serum, liter tissue, and feces in rats. After one week of general diet feeding, four week old S.D. rats were fed basal group, cholesterol diet group (1$\%$ cholesterol), and rolymannuronate diet group (1$\%$ cholesterol,5$\%$ polymannuronate). The total cholesterol levels in the serum and liver tissue was significantly decreased in the polymannuronate diet group. The polymannuronate diet group showed increased amounts of feces, total fecal bile acid and dietary fiber contents compared to basal diet group and cholesterol diet groups. Also, the histology of liver showed serious localized fat drops in the cholesterol diet group, but the polymannuronate diet group showed fewer localized fat drops. These results suggest that feeding of polymannuronate improves the lipid metabolism profile of rats by changing the serum and liver tissue cholesterol levels with increased level of the total bile acid, total dietary fiber and total cholesterol level in the feces.