The goal of refining is to treat fibers so they meet the requirements of the papermaking process. The refining process in papermaking has great influence on the quality of the final product by changing the fiber properties, such as fiber length, shape, fine contents and so on. In this study, the effect on the morphological change of fibers by the refining conditions were investigated using the fiber morphology analyzer. Fiber morphology analyzer used to determine which pulps are suitable for producing particular products. Furthermore it is widely used in paper mills to monitor paper quality. The morphological change of fibers according to refining conditions were evaluated out by measuring fiber, shive and fine. In the fiber morphology, the domestic reconstituted tobacco fiber has the bigger average fiber length value than that of the foreign reconstituted tobacco.