Levels of fumonisins, mycotoxins produced by fungal species, must be accurately and rapidly monitored to ensure food safety. In this study, using surface plasmon resonance sensor, a batch-type biosensor was fabricated to detect fumonisin $B_1$. By applying this biosensor to fumonisin $B_1$ solutions of 0 to 6 ppm, a significant calibration model was developed for measurement. Coefficient of determination in regression analysis for the model was 0.920. Results indicate that detection of fumonisin $B_1$ by surface plasmon resonance biosensor was highly feasible.