Objectives: This experimental study was carried out to evaluate the immune modulating and anti-tumor activity of Ampelopsis Radix extracts (ARE). Materials and Methods: To elucidate the effects of ARE on the macrophage and NK cell activity, we analyzed NO production, NK cytotoxicity and gene expressions of cytokine related with macrophage and NK cell activity. Results: ARE activated and promoted macrophages to product NO in part. And, ARE has significant properties to activate macrophages and NK cells by promoting related cytokines like IL-1, IL-12, IFN-$gamma$, iNOS and TNF-$alpha$ gene expressions. We also observed that ARE promoted protein expression of IFN-$gamma$, and TNF-$alpha$ in mice splenocytes. Conclusions: ARE is an effective herbal drug for immune modulating and anti-cancer by promoting activity of macrophages and NK cells.