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Isolation of Protease-Producing Arctic Marine Bacteria
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  • Isolation of Protease-Producing Arctic Marine Bacteria
  • Isolation of Protease-Producing Arctic Marine Bacteria
저자명
Lee. Yoo-Kyung,Sung. Ki-Cheol,Yim. Joung-Han,Park. Kyu-Jin,Chung. Ho-Sung,Lee. Hong-Kum
간행물명
Ocean and polar research
권/호정보
2005년|27권 2호|pp.215-219 (5 pages)
발행정보
한국해양연구원
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

We isolated and identified three protease-producing bacteria that had inhabited the region around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway $(79^{circ}N,;12^{circ}E)$. Biofilms were collected from the surface of a floating pier and from dead brown algae in a tide pool near the seashore. The biofilm samples were transported to the Korea Polar Research Institute (KOPRI) under frozen conditions, diluted in sterilized seawater, and cultured on Zobell agar plates with 1% skim milk at $10^{circ}C$. Three clear zone forming colonies were selected as protease-producing bacteria. Phylogenetic analysis based on 16S rDNA sequences showed that these three stains shared high sequence similarities with Pseudoalteromonas elyakovii, Exiguobacterium oxidotofewm Pseudomonas jessenii, respectively. We expect these Arctic bacteria may be used to develop new varieties of protease that are active at low temperatures.