Water content of sub-layer in pavement systems has a large effect on pavement performance. Many researchers lately make use of time-domain reflectometry(TDR) probes to measure the soil water content of sub-layer from field monitoring. The laboratory calibration test of TDR probe should be performed with soil field, because TDR probe can cause an error by type, gradation, density, and temperature of soil. This study performed the laboratory calibration test of TDR probe(CS616) with subgrade and subbase material in long term pavement performance(LTPP) sections. And the calibration equations of TDR probe(CS616) were then proposed. It was confirmed from the study that the data of TDR probe monitored in field could be used to estimate the freezing, unfrozen water content, and matric suction of soil.