The occurrence and abundance of protozoa at advanced wastewater treatment plant were compared with operating parameters and effluent quality using statistical procedures. In correlation analysis between the distribution of protozoa and operating parameters, the distribution of protozoa was showed the operating condition of plant. Regression analysis between the distribution of protozoa and effluent quality up to 7 days, showed the R-square values of most regression equation were more than 0.6 and constant was higher than slope and could indicate effluent quality from sampling day to 7 days. Once enough data concerning protozoa, operating parameters and effluent has been gathered, the operator has a valuable tool for predicting plant performance and near-future effluent quality based on microscopic examination. Plant operator manipulates operating conditions if he knows near-future data of effluent is deteriorating. Perhaps more importantly it can be used to actually control the plant to adjust the operating conditions to obtain the protozoal populations that have been shown to provide the best effluent quality.