The purpose of this study was to compare the differences of hip and thigh muscle activities between subjects with increased and decreased femoral anteversion during stair ascent. Twelve healthy female volunteers participated in this study. The subjects were divided into two groups (group 1 with increased anteversion of the hip, group 2 with decreased anteversion of the hip). This study analyzed differences in each mean peak gluteus maximus (GM), gluteus medius (GD) and tensor fascia lata (TLF) EMG amplitude: composite mean peak hip muscles (GM, GD, TFL) EMG amplitude ratios and in each mean peak vastus medialis oblique (VMO), vastus lateralis (VL), biceps femoris (HM) and semitendinosus (HL) EMG amplitude: composite thigh muscles (VMO, VL, HM, HL) EMG amplitude ratios among subjects with decreased or increased relative femoral anteversion. EMG ratios were compared in the stance and swing phase of stair ascent. Group 1 showed an increased standardized mean GM and GD EMG amplitude and decreased standardized mean TFL to composite mean hip muscles EMG amplitude ratios in stair ascent during both stance and swing phase. Also, group 1 showed an increased standardized mean HL EMG amplitude and decreased standardized mean VL and HM to composite mean thigh muscles EMG amplitude ratios in stair ascent during both stance and swing phases. There was no statistically significant difference in vastus medialis oblique between subjects with increased or decreased relative femoral anteversion. In order to provide rehabilitation professionals with a clearer picture of the specific requirements of the stair climbing task, further research must be expanded to include a wider range of age groups that represent the general public, such as including middle-aged healthy persons.