In this study, ground reaction force(GRF), absolute symmetry index(ASI) and coefficient of variation(CV) of fixed, single-axis and multi-axis prosthetic ankle assemblies were investigated to show the biomechanical evaluation for above knee amputees. In the experiments, 37 normal male volunteers, two male and two female AK amputees were tested with fixed, single-axis and multi-axis prosthetic ankle assembly. A gait analysis was carried out to derive the ratio of GRF to weight as the percentage of total stance phase for ten points. The results showed that fixed-axis ankle was superior to the other two ankle assemblies for the characteristic of forwarding and breaking forces. Multi-axis ankle was relatively superior to the other two ankle assemblies for gait balancing and movement of the center for mass. single-axis ankle was relatively superior to the other two ankle assemblies for CV and ASI of GRF.