This study is intended to find out the reason of interference phenomenon of a machine tool when it operates for 5-axises working. The researcher made a Virtual Machine which has same figures of the 5 axises machine tool and Virtual Manufacturing System which has both Software factors - controller and NC code data to manipulate the movement characteristics of the machine - and Hardware factors - fixtures, workpiece, tools, holders and so on. With these virtual tools, this study is designed to find out the relation between the movement and the interference or collision, and also intended to verify the simulation and work-processing. In this study, the researcher found out, in case of the vertical 5 axises type, that it has more chances to have interference between the fixture, the workpiece and the main spindle including the tool holder due to the tilting kinetics of the main spindle. In case of the horizontal 5 axises type, on the other hand, the researcher found out that it has more possibility to have the interference between the main spindle and the rotary shaft.