Objectives. The purpose of this study was to compare the shade changes in wet and dry conditions of natural teeth using two different intra-oral colorimeters. Materials and methods. Twenty volunteer subjects have no restorations and fillings in the maxillary central incisors were involved in this clinical study. The color of tooth was measured by two different instruments that were a Shade $Scan^{TM}$ System and a VITA $Easyshade^{(R)}$, Five times consecutive measurements were done for each subject with both instruments. Groups of measurement are an initial wet condition as control, dry in 5 minutes, 15 seconds after re-wetting with saliva, re-wetting after 5minutes and re-wetting after 30 minutes. Using ShadeScan $System^{TM}$, tooth image was captured and converted to the mapping image of Vitapan 3D master. Three main shades were chosen from each subject and calculated the area in Global Lab Image software. Data were analyzed using paired T-Test and Wilcoxon Signed Ranked Test. Using VITA $Easyshade^{(R)}$, color differences($Delta$E) between measurements were analyzed with one sample T-test. Results. Using ShadeScan $System^{TM}$, there were significant differences between control group and dry(P=.023), dry and re-wetting 15 seconds, 5 minutes, 30 minutes as well(P=.021, P=.017, P=.030) in comparison of primary shade. However, comparing three main shades, there was no significant difference between control and dry(P=.105). Using VITA $Easyshade^{(R)}$, color differences($Delta$E) between control and dry, dry and re-wetting 30 minutes were statistically different(P=.002, P=.022). Conclusion. Primary shade could be changed in dry and wetting procedure in time, however there was no significant shade changes in overall.