Statement of problem. Initial stability of implant is an important factor for predicting osseointegration. It requires a rapid, non-invasive, user-friendly technique to frequently assess the implant stability and the degree of osseointegration. Purpose. The aim of this study was to evaluate the correlation between the resonance frequency analysis (RFA) and the radiographic method for peri-implant bone change under in vitro conditions. Material and Method. Twenty implants of 3.75 mm in diameter(Neoplant, Neobiotech, Korea) were used. To simulate peri-implant bone change, 2 mm-deep $45^{circ}$ range horizontal defect and 2 mm-deep $90^{circ}$ range horizontal defect area were serially prepared perpendicular to the X-ray beam after conventional implant insertion. Customized film holding device was fabricated to standardize the projection geometry for serial radiographs of implants and direct digital image was obtained. ISQ values and gray values inside threads were measured before and after peri-implant bone defect preparation. Results. Within a limitation of this study, ISQ value of resonance frequency analysis was changed according to peri-implant bone change (p<0.05) and gray value of radiographic method was changed according to peri-implant bone change (p<0.05). There was no correlation between the ISQ value and the gray value for peri-implant bone change (p>0.05). But, in horizontal defect condition, relatively positive correlation were between ISQ and gray values(r=0.663). Conclusion. This results provided a possibility that peri-implant bone change may be evaluated by both RFA and radiographic method.